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Abstract:
In this study, the conditions of the enzymatic hydrolysis for Limonia acidissima (L. acidissima) pulp with the combination of cellulase and pectinase were identified. The interations of pH and temperature, the concentration of the combined enzymes and the hydrolysis time in the hydrolysis process effective affected on the total dry matter and the antioxidants recovery. The best hydrolysis conditions were established at pH of 4.2, temperature of 45oC, the concentration of the combined enzymes of 1.2 percent of volume per dry weight basic (% v/dwb) and the hydrolysis time of 90 min. As a result, the recovery efficiency of the total dry matter was highest, that increased 20% in comparison with the efficiency from the non-enzymatic extraction process. Besides, the antioxidants included total phenolic content (TPC), DPPH and ABTS radical scavenging activity increasing from 451.45+_3.55 to 704.92+_10.25 mg gallic acid equivalent in a liter (GAE/L), 0.93+_0.01 to 1.52+_0.05 and 1.08+_0.06 to 2.02+_0.07mM trolox equivalent antioxidant capacity (TEAC) respectively. That might promise producing many kind of foods from the extract of the the pulp, which provide many health benefits.
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